Journal: Developmental cell

Article Title: Single Cell Profiling Reveals Sex, Lineage and Regional Diversity in the Mouse Kidney

doi: 10.1016/j.devcel.2019.10.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-Calb1 , Sigma , Cat# C9848; RRID: AB_476894.

Techniques: In Situ Hybridization, Recombinant, Multiplex Assay, RNA Sequencing Assay, Software, Real-time Polymerase Chain Reaction, Microscopy, Flow Cytometry

Journal: Neuroscience Bulletin

Article Title: Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus

doi: 10.1007/s12264-019-00362-z

Figure Lengend Snippet: Mutant SFV VLP rapidly and sparsely labels Purkinje neurons in vivo. To sparsely label Purkinje cells, the mutant virus was injected into the third cerebellar lobule (3Cb) region. The mice were sacrificed at 24 hpi and sagittal and coronal sections were prepared. A The EGFP signals were co-localized with the Purkinje cell marker calbindin-D28k (arrows). B The fine structure of the Purkinje cells was apparent, including the dendritic tree, long axon, and axonal branches (asterisks). These images are representative of 15 sections (n = 3 mice).

Article Snippet: To stain Purkinje cells, fixed sagittal sections 80 μm thick were immunostained with a mouse monoclonal antibody against calbindin-D28k (Proteintech) and amplified with a Cy3-conjugated anti-mouse secondary antibody (Merck).

Techniques: Mutagenesis, In Vivo, Virus, Injection, Marker

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: (a) White arrows indicate the localization of IBV N protein in AQP2-expressing collecting duct cells. Green fluorescence shows positive staining for IBV N and red fluorescence shows staining for AQP2. (b) White arrows indicate the localization of IBV N protein in CALB1-expressing distal tubule cells. Red fluorescence shows positive staining for IBV N and green fluorescence shows staining for CALB1. (c) Changes in cell communication numbers: The top network diagram shows cell clusters as nodes, with line thickness indicating changes in communication numbers. The lower heatmap details these changes, with rows representing signal-sending cells and columns indicating signal-receiving cells. The color scale reflects the inter-group differences in signal communication frequency between different cell types (number of communications in the infected group—number in control group). The bar plots at the top and right side represent the overall differences in the number of signals sent/received by specific cell clusters. (d) Changes in cell communication strength: Similar to (c), with the top network diagram displaying changes in communication strength (communication strength in the infected group—strength in the control group). In the lower heatmap, the color scale reflects the inter-group differences in signal communication strength between different cell types. The bar plots at the top and right side represent the overall differences in the strength of signals sent/received by specific cell clusters (infected group—control group). (e) Inter-group differences in the communication strength of specific signaling pathways (receptor-ligand pairs) across cell clusters. Rows represent signal pathways and columns correspond to cell clusters, with heatmap colors depicting the strength variation of signals (infect group vs. control group). Upper left triangles for signal sent and lower right triangles for signal received. The bar plot on the right side shows the overall difference in communication strength of these signaling pathways between the IBV-infected group and the control group.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Expressing, Fluorescence, Staining, Infection, Control, Protein-Protein interactions

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: Antbodies and reagents used in present study.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Blocking Assay, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca 2+ Levels in Senescent Cells

doi: 10.3390/ijms23169376

Figure Lengend Snippet: CALB1 overexpression buffers increased Ca 2+ levels induced by oncogene activation in HMECT. ( A ) CALB1 protein level was assessed by Western Blot in HMECT-Mek:ER (without 4-OHT) infected with a vector encoding CALB1 or the corresponding GFP control vector. α-Tubulin protein level was assessed as a loading control. ( B ) Ca 2+ levels were measured with the ratiometric probe Fura2 in HMECT-Mek:ER inducible oncogene and CALB1 or the corresponding GFP control vector, with or without 4-OHT treatment. Resting cytosolic Ca 2+ concentration was evaluated as a stable fluorescent ratio before stimulation ( left panel), and the size of Ca 2+ intracellular stocks was estimated as the Ca 2+ peak amplitude obtained after ionomycin stimulation ( right panel). Means +/− SEM of 3 independent experiments are presented. Kruskal–Wallis test was performed, and p -values are indicated. Three days after 4-OHT treatment, GFP- 4-OHT n = 85 or + 4-OHT n = 126, CALB1- 4-OHT n = 99 or + 4-OHT n = 77.

Article Snippet: An anti-CALB1 rabbit polyclonal antibody (sc28285, Santa Cruz, Santa Cruz, CA, USA) and anti-α-tubulin mouse monoclonal antibody (T6199, Sigma-Aldrich) were used.

Techniques: Over Expression, Activation Assay, Western Blot, Infection, Plasmid Preparation, Concentration Assay